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      Per 23-3-2018 the ISBT Forum is temporarily closed. We are in the process of setting up a new forum within the MyISBT section of the ISBT website. We are happy to welcome you back on the new ISBT Forum soon. Please check your email for an update once the new Forum is available. Thank you for your understanding.

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Merhan Fouda

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About Merhan Fouda

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  • Birthday February 20

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  1. Crossmatch tube top colour

    In Egypt- EDTA tubes (purple top)
  2. When to check Hb after blood transfusion?

    This was a Q&A on CAPs website: Q. How long should you wait after a unit of blood has been transfused before drawing a complete blood count, or doing other lab work, to ensure accurate test results? A. Optimum timing of post-transfusion phlebotomy is critical for ensuring meaningful laboratory testing results, and medical judgment is required in making this determination. Several factors must be considered, including the type and amount of blood product given, purpose of the test (that is, the question it is intended to answer), and clinical setting. In general, it is best to perform phlebotomy when the patient’s circulatory system is in homeostasis. A patient who is bleeding or undergoing blood product transfusion, or both, is not in a steady state. Whenever possible, samples for laboratory testing should be postponed until bleeding has stopped and transfusion is complete. One obvious exception to this rule, however, would be the setting of massive transfusion, during which monitoring certain laboratory values, such as cell counts and coagulation parameters, is essential to guide ongoing therapy. Variables such as patient blood volume, cardiac output, renal function, and volume of blood products transfused affect how quickly homeostasis is achieved following transfusion. For the evaluation of post-transfusion increments in hemoglobin, hematocrit, and platelet counts, a practical approach is to draw blood samples within 10 to 60 minutes after completing transfusion, as this time interval is aimed at measuring peak recovery.1 Results determined from blood samples drawn later than 60 minutes post-transfusion are increasingly affected by confounding conditions, such as splenic sequestration, sepsis, and consumption.1,2 If the intent is to determine the extent of such confounding processes on red cell and platelet counts, one should combine a 10-minute post-transfusion sample with sequential samples drawn at one hour and 24 hours post-transfusion. Alterations in chemistry test results following transfusion are not usually a concern in the low-volume transfusion setting. However, assay results may be affected for varying periods following transfusion of large amounts of blood products, as seen in massive transfusion, red cell, or plasma exchange—particularly if the recipient has impaired hepatic or renal function. Banked storage of red cells results in elevated plasma levels of hemoglobin, potassium, LDH, and iron in the blood unit that may, particularly in the metabolically impaired patient, be reflected in the post-transfusion laboratory values. In addition, citrate anticoagulant present in blood products may result in transient hypocalcemia in the recipient.3 Therefore, following large-volume transfusions or exchanges, waiting 12 to 24 hours before drawing samples for chemistry assays will provide results that are more reflective of the patient’s underlying metabolic state. References Choo Y. The HLA system in transfusion medicine. In: McCullough J, ed. Transfusion Medicine. New York, NY: *McGraw–Hill Book Co;1998:401. Legler TJ, Fischer I, Dittman J, et al. Frequency and causes of refractoriness in multiply transfused patients. Ann Hematol. 1997;74:185–189. Brecher ME, ed. Technical Manual. 15th ed. Bethesda, Md.:AABB;2005;649–650. Other Articles: Elizalde JI, et al.Early changes in hemoglobin and hematocrit levels after packed red cell transfusion in patients with acute anemia. Transfusion. 1997 Jun;37(6):573-6 Wiesen AR, et al. Equilibration of Hemoglobin Concentration after Transfusion in Medical Inpatients Not Actively Bleeding. Ann Intern Med. 1994;121:278-280
  3. Happy Birthday Linley with many happy returns.:)

  4. Getting to know each other

    Hello Everyone. I am Merhan Fouda. I am a lecturer in Clinical Pathology Department (Hematology and Blood bank unit) at the National Cancer Institute, Cairo University in Egypt. This is a great initiative by the ISBT to bring all the transfusion medicine community together in a single platform; to know each other, share knowledge and exchange experience. Wishing you all a Happy New Year.